Fig. 6: NAD dyshomeostasis resulted from insect infestation activates jasmonate biosynthesis for plant defense priming.
From: NAD+ deficiency primes defense metabolism via 1O2-escalated jasmonate biosynthesis in plants

a A schematic for the metabolite analysis of plant under insect infestation. The contents of NAD+ (b), NADH (c), NADP (d) and NADPH (e) in rosette leaves of Col-0 wild-type plant under insect infestation. Each bar represents the means ± SD (n = 4 biological replicates), and the significance analysis was performed by two-sided Student’s t test. f Determination of 1O2 level in rosette leaves of Col-0 wild-type plant under insect infestation for indicated hours. n = 5, two-sided Student’s t test. The contents of JA (g) and JA-Ile (h) in the leaves of Col-0 under insect infestation. The data represent the means ± SD (n = 4), two-sided Student’s t test. i A proposed model showing the activation and operation of NAD+-depletion defense pathway in response to biotic stress. Source data are provided as a Source Data file. a and i are created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en).