Fig. 3: Structural characterization of sequence-controlled BSA-multiblock polymer conjugates. | Nature Communications

Fig. 3: Structural characterization of sequence-controlled BSA-multiblock polymer conjugates.

From: Sequence-encoded bioactive protein-multiblock polymer conjugates via quantitative one-pot iterative living polymerization

Fig. 3

a In situ monitoring of ATRP progress of three acrylates (MA, M′; OEGA, O′; HEA, H′) initiated with PEG2K-Br using 1H NMR spectrometry. The quantitative conversion was confirmed with the disappearance of the vinyl signals at 5.8–6.4 ppm. b, c SEC traces of all growing BSA-multiblock polymer conjugates via QOILP, including (b) all bioconjugate intermediates towards BSA-HHHHHH, and (c) all bioconjugate intermediates towards BSA-MOHMOH. d SEC traces of BSA, BSA-Macro, and six target sequence-controlled BSA-multiblock polymer conjugates. e SDS-PAGE of BSA, BSA-Macro, and six target sequence-controlled BSA-multiblock polymer conjugates. Representative images from three independent experiments are shown. f CD spectra of BSA and sequence-controlled BSA-multiblock polymer conjugates. g Biochemical stability assay of BSA and BSA-multiblock polymer conjugates after heating to 90  °C for 30 min by HPLC (n = 3). h Esterase-like activities of BSA and BSA-multiblock polymer conjugates. The data are shown as mean ± SD (n = 3) for g and h. Source data are provided as a Source Data file.

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