Fig. 5: The in vitro cytotoxicity and cellular uptake of BSA-multiblock polymer conjugates by L929 cells. | Nature Communications

Fig. 5: The in vitro cytotoxicity and cellular uptake of BSA-multiblock polymer conjugates by L929 cells.

From: Sequence-encoded bioactive protein-multiblock polymer conjugates via quantitative one-pot iterative living polymerization

Fig. 5

a The illustration of the sequence-dependent cellular-uptake process. b Cytotoxicity assay of BSA and sequence-controlled BSA-multiblock polymer conjugates incubated with L929 cells for 24 h. c, d Cellular uptake of Cy5.5-labeled BSA and Cy5.5-labeled BSA-multiblock polymer conjugates by L929 cells detected by flow cytometry for (c) 30 min and (d) 4 h. Insert: Quantitative fluorescence (FL) intensity comparisons based on the fluorescence-activated cell sorting (FACS). e Confocal images of L929 cells after incubation with Cy5.5-labeled BSA and Cy5.5-labeled BSA-multiblock polymer conjugates for different times (blue: DAPI, red: Cy5.5). Representative images from three independent experiments are shown. The data are shown as mean ± SD (n = 4) for b and the data are shown as mean ± SD (n = 3) for c and d. Figure 5a Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license. Source data are provided as a Source Data file.

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