Table 1 Overview on applied omics technologies
From: A roadmap to the molecular human linking multiomics with population traits and diabetes subtypes
Omics | Measurement/Output | Technique/Platform | Matrix | Label |
|---|---|---|---|---|
GENOMICS | Genotype | Infinium Human Omni 2.5–8 v1.2 BeadChip kit | DNA extracted from buffy coat fraction from whole blood | DNA |
METHYLOMICS | DNA methylation | Illumina Infinium HumanMethylation450 BeadChip kit | DNA, same as for genomics | MET |
TRANSCRIPTOMICS | Gene expression | RNA-sequencing based Illumina ~20 M reads | RNA extracted from PaxTube | RNA |
microRNA expression | microRNA profiling based multiplex qPCR, Exiqon | RNA extracted from EDTA plasma | miRNA | |
PROTEOMICS | Relative protein abundance | Slow Off-rate Modified Aptamer (SOMAmer), Somalogic 1,1k | EDTA plasma | SOMA |
Relative protein abundance | Proximity Extension Assay (PEA) based Olink Target 96 Metabolism & Cardiometabolism panels | Heparin plasma | OLINK | |
GLYCOMICS | Total plasma N-glycosylation | Hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) based Genos pipeline | EDTA plasma | PGP |
IgG glycosylation | Liquid chromatography mass spectrometry (LC-MS) based Genos pipeline | EDTA plasma | IgG | |
IgA & IgG glycosylation | LC-MS based124 pipeline | EDTA plasma | IgA | |
LIPOPROTEOMICS | Lipoproteins | Proton nuclear magnetic resonance (1H NMR) based Nightingale technology | EDTA plasma | BRAIN |
LIPIDOMICS | Absolute lipid concentration | LC-MS based on Lipidyzer technology at Metabolon | EDTA plasma | LD |
Lipids and other metabolite concentration | Flow injection analysis (FIA)- MS based Biocrates technology | EDTA plasma | BM | |
METABOLOMICS | Metabolite level | (HILIC-MS) & (UPLC-MS) based HD4 Metabolon | EDTA plasma | HDF |
Metabolite level | Gas chromatography (GC)-MS (UPLC-MS) based HD2 Metabolon | EDTA plasma | PM | |
Metabolite level | (GC-MS) & (UPLC-MS) based HD2 Metabolon | Saliva | SM | |
Metabolite level | GC-MS & UPLC-MS based HD2 Metabolon | Urine | UM | |
Metabolite level | 1H NMR deploying Chenomx for annotation, based on131 pipeline | Urine | CM | |
CLINICAL | Clinical biochemistry and blood counts | Cobas 6000; Roche Diagnostics | Blood/Urine | CLIN |
