Fig. 2: Factors affecting the PenG-specific serological response. | Nature Communications

Fig. 2: Factors affecting the PenG-specific serological response.

From: Covalent penicillin-protein conjugates elicit anti-drug antibodies that are clonally and functionally restricted

Fig. 2

a Sex-matched C57BL/6 mice were immunized three times and the serological response at the terminal timepoint (wk 10) was evaluated. b, c Mice were immunized three times (wk 0, 4 and 8) and the terminal (wk 10) IgG PenG-specific EPTs were evaluated. d Mice were bled and 2 mg/mL PenG was added and mixed end-to-end overnight. Animals were subsequently immunized (wk 0 and 4) intravenously with seum-PenG with or without alum. e, f Terminal IgG EPTs were evaluated. b, c, e, f Dots represent data from a single animal (n = 4–8 per group), and bars/text denotes the median (ND = not detected). Data were compared via a Kruskal–Wallis test (two-sided). g PenG-Ben structure. h Mice were given PenG-Ben intramuscularly. i PenG-specific IgG titres were evaluated. Dots represent data from a single animal (n = 27). Data were compared to pre-administration, evaluating the ratio of responders via Chi-squared test. a, d, h Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license. Source data are provided as a Source Data file.

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