Fig. 4: Immunogenetic characterisation of the PenG-specific response. | Nature Communications

Fig. 4: Immunogenetic characterisation of the PenG-specific response.

From: Covalent penicillin-protein conjugates elicit anti-drug antibodies that are clonally and functionally restricted

Fig. 4

a Immunisation schedule. Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license. b Antigen probe sorting strategy on pre-gated non-naïve B cells (DUMP-B220+IgD-). c Inferred clonal families from the PenG probe-sorted B cells (coloured) and singletons (grey). d Inferred VH gene segment across the sequenced B cells. Bar segment sizes proportional to the number of B cells of the same VH origin. Joining of the segments connote the shared utilisation between mice. Segment colours reflect the IGHV subgroup. e Logo plots of the CDRH1 and CDRH2 amino acid sequences from all sequenced B cells in all animals. f Fab binding specificity assay setup. g ELISA trace for a subset of 28 Fabs and (h) their OD1.25 intersection (estimate for EC50) values. BAR-1 (grey) is an unrelated negative control specific to sialyllactose. Dots represent data from a single Fab clone (n = 28). Source data are provided as a Source Data file.

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