Fig. 2: HMGA1 leads to global re-organisation of chromatin architecture in OIS. | Nature Communications

Fig. 2: HMGA1 leads to global re-organisation of chromatin architecture in OIS.

From: HMGA1 orchestrates chromatin compartmentalization and sequesters genes into 3D networks coordinating senescence heterogeneity

Fig. 2

a Hi-C interaction maps (resolution 200 kb) of Grow, Grow-shA1, OIS, and OIS-shA1 conditions, with H3K9me3 peaks marked (blue) and arcs representing increased (red) and decreased (blue) interactions. b Number of significantly increased (Inc) and decreased (Dec) interactions in OIS-shA1 compared to OIS, crosschecked against the changes in OIS compared to Grow. c The number of OIS-shA1 compared to OIS interaction changes where one or both regions involved is bound by HMGA1 (minimum 5 peaks per bin). d The distance between the regions involved in differential interactions against the interaction log-fold changes in OIS compared to Grow (left) and OIS-shA1 compared to OIS (right); the vertical dashed line marks the 2 Mb distance threshold denoting distal interactions. e Log-fold changes of the differential interactions between A-compartment regions (AA), B-compartment regions (BB) and between A- and B-compartment regions (AB), categorised by local (within 2 Mb) and distal (>2 Mb) interactions, in the OIS compared to Grow (purple) and OIS-shA1 compared to OIS (orange) comparisons. f Differential interaction networks of chromosome 4 between nodes representing 200 kb bins, coloured by H3K9me3 status, and edges representing increased (red) and decreased (blue) interactions; left: OIS compared to grow and right: OIS-shA1 compared to OIS; nodes are positioned according to the Fruchterman-Reingold layout calculated based on the edge weights representing the increased interaction log-fold changes.

Back to article page