Fig. 3: Shn3 depletion in early-stage osteoblasts but not adipose tissue produces anti-obesity effects. | Nature Communications

Fig. 3: Shn3 depletion in early-stage osteoblasts but not adipose tissue produces anti-obesity effects.

From: Bone controls browning of white adipose tissue and protects from diet-induced obesity through Schnurri-3-regulated SLIT2 secretion

Fig. 3

a Body weight curve of male Shn3OSX and Shn3f/f mice after HFD for 12 weeks (n = 10 for each group, P values directly labeled above the time points). b Body weight curve of Shn3Adipoq and Shn3f/f mice (n = 10) after HFD for 12 weeks. c Tissue wet weight of iWAT, eWAT, BAT and liver (Shn3OSX and Shn3f/f, upper, n = 10 for Shn3f/f group and n = 8 for Shn3OSX group. Shn3Adipoq and Shn3f/f, lower, n = 8 for Shn3f/f group and n = 7 for Shn3Adipoq group.). d Representative histological sections of iWAT, eWAT and liver from 3 slides of Shn3OSX and Shn3f/f mice, respectively. Scale bar=200 um. e–f Results of intraperitoneal glucose tolerance and insulin tolerance tests (P values directly labeled above the time points) and the area under the curve for Shn3Osx and Shn3f/f mice is shown (n = 6 for each group). g Serum C-peptide level measured by sandwich ELISA of Shn3Osx (n = 8) and Shn3f/f (n = 10) individuals. h Representative H&E and insulin IHC of pancreas islet from 3 individual slides. Scale bar=200 um. i–j Histomophometric quantification result of islet area/tissue area and islet number (n = 5 for each group). (k) Quantification of serum triacylglycerol (TG, n = 7 for Shn3Osx group and n = 8 for Shn3f/f group), free fatty acid (FFA, n = 8 for each group) level and adiponectin (n = 8 for each group) of Shn3OSX and Shn3f/f mice through ELISA assays. l Representative H&E sections (Scale bar=500 um) and Ucp-1 IHC results (Scale bar=200 um) of iWAT from 3 individual slides of Shn3Osx and Shn3f/f individuals, respectively. m Representative immunoblots of UCP1 of iWAT obtained from HFD-fed Shn3Osx and Shn3f/f mice. n Quantification of Ucp-1 expression level in iWAT from HFD-fed Shn3Osx (n = 7) and Shn3f/f (n = 8) mice. o Thermogenic gene expression level relative to Gapdh in iWAT of Shn3Osx (n = 10) and Shn3f/f mice (n = 7). p Lipolytic gene expression in iWAT harvested from HFD-fed Shn3Osx and Shn3f/f mice (n = 5 for each group). Results were presented as mean ± s.d., analyzed by unpaired, two-tailed Student’s t test (lower of c, g, i–k, n–p), one-way ANOVA with Tukey’s test (upper of c), two-way ANOVA with Bonferroni’s multiple comparisons test (a, b, e, f). *P < 0.05, **P < 0.005, ***P < 0.0005, ****P < 0.0001 (Shn3f/f vs. Shn3Osx). #P < 0.05, ##P < 0.005, ###P < 0.0005 (Osx Cre vs. Shn3Osx). Source data are provided as a Source Data file.

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