Fig. 4: Skeletal Shn3-ablation augments adipose tissue browning in an endocrine manner. | Nature Communications

Fig. 4: Skeletal Shn3-ablation augments adipose tissue browning in an endocrine manner.

From: Bone controls browning of white adipose tissue and protects from diet-induced obesity through Schnurri-3-regulated SLIT2 secretion

Fig. 4

a Schematic diagram of the co-culture assay. b Thermogenic gene expression in co-cultured differentiated beige adipocytes (n = 4 for each group). Genotypes of osteoblasts are indicated. Results were collected from 3 repeated experiments. c Schematic diagram of transplantation assay. d Graft weight of fat graft transplanted into Shn3Osx and Shn3f/f hosts. e Representative H&E sections and UCP-1 IHC results of fat allografts implanted into Shn3Osx and Shn3f/f hosts for 4 weeks. respectively. Results were collected from 3 individual slides. f Ucp1 gene expression level relative to Gapdh of fat graft transplanted into Shn3Osx and Shn3f/f hosts (n = 4 for each group). Results were presented as mean ± s.d., analyzed by unpaired, two-tailed Student’s t test (b, d, f). Diagram (a) and (c) were generated through utilizing BioRender Apps. Source data are provided as a Source Data file.

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