Fig. 6: AAV-9 mediated SHN3 depletion protected mice from development of DIO. | Nature Communications

Fig. 6: AAV-9 mediated SHN3 depletion protected mice from development of DIO.

From: Bone controls browning of white adipose tissue and protects from diet-induced obesity through Schnurri-3-regulated SLIT2 secretion

Fig. 6

Representative IVIS imaging of (a) whole body and (b) major organs from murine models after the injection of a single dose of 4 × 1011 GC rAAV9-amiR-Shn3 or rAAV9-amiR-Ctrl (Fluorescence signal intensity labeled as colored symbols on the right. Yellow represents strong signal and red represents dim signal). μCT reconstruction and quantification of (c) femoral trabecular and (d) cortical bone of rAAV9-amiR-Shn3 or rAAV9-amiR-Ctrl administered mice (n = 5 for each group). Scale bar=1 mm. e Body weight curve of rAAV9-amiR-Shn3 and rAAV9-amiR-Ctrl injected mice over a 12-week HFD exposure (n = 8 for each group). f General view of inguinal adipose fat pad and intrascapular brown adipose tissue. g Tissue wet weight of iWAT, eWAT, liver and BAT obtained from rAAV9-amiR-Shn3 and rAAV9-amiR-Ctrl injected mice (n = 8 for each group). h Representative histological H&E results of iWAT and liver from rAAV9-amiR-Shn3 and rAAV9-amiR-Ctrl group. Scale bar=100 um. Results were selected from 3 repeated experiments. i Thermogenic gene expression level relative to Gapdh in iWAT of mice treated with rAAV9-amiR-Shn3 or rAAV9-amiR-Ctrl (n = 6 for each group). j IGTT and ITT results at 10 weeks after rAAV injection (n = 8 for each group). Results were presented as mean ± s.d., analyzed by unpaired, two-tailed Student’s t test (c–d, g, i) or two-way ANOVA with Bonferroni’s multiple comparisons test (e, j). *P < 0.05, **P < 0.005, ***P < 0.0005, ****P < 0.0001. Source data are provided as a Source Data file.

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