Fig. 1: Molecular interactions of the ARM-1—AS-7 co-culture.
From: Cell-to-cell interactions revealed by cryo-tomography of a DPANN co-culture system
A Representative tomographic slice of ARM-1 (three smaller cells) cells interacting with their host AS-7 (large single cell, top). White boxes highlight the interacting interfaces of ARM-1 and AS-7, and red stars indicate the location of tube structures adjoining the two organisms. B Segmentation analysis of the 3D volume highlights key components: tube-like structures (white), host S-layer (red), host inner membrane (pink), ribosomes (yellow), ARM-1 S-layer (dark blue), and ARM-1 cytoplasmic membrane (light blue). C Enlarged view of another ARM-1 cell (centered) interacting with the AS-7 host (top right). Views of tube-like structures are highlighted by a red box, inter-cell connecting tubes (red arrows), and the side-view of a primed tube within the AS-7 membrane envelope (yellow arrows) are clearly visible. D Top view of an AS-7 cell a red dashed circle highlights the top view of a primed tube in the S-layer. E Two side views of the host AS-7 membrane and S-layer, thin protrusions are shown (white arrows) which are a different structure to the tubes (yellow arrow). Images in A, C–E are 2D slices through 3D reconstructed volumes generated from a tilt series of 2D projection images. Scale bars indicate 100 nm A, 50 nm (C–E).
