Fig. 7: MINSTED fluorescence nanoscopy following two-photon activation of ONB-2SiR in samples of immunostained caveolin-1 in U-2 OS cells.

a Confocal image (counter-stain with Alexa Fluor 647). b MINSTED reconstruction (median localization precision ~2 nm). c Enlarged views of caveolin-1 distributions. Scale bars: 300 nm a, b, 50 nm c.