Fig. 6: LTα₁β₂ expression regulates T_reg glucose dependence during their development.

a, b Representative histogram and fold change in the gMFI of MitoTracker Deep Red (MDR) staining in CCR6⁻ CD25⁺ T_regP, Foxp3^lo T_regP and mature T_reg from Foxp3^eGFP (n = 9) mice (a) compared to Foxp3^eGFPxLta^−/− (n = 9) mice (b). FMO are shown in Foxp3^eGFP T_reg subsets. Data are pooled from two independent experiments. c, d SCENITH metabolic profiling of CD25⁺ T_regP, Foxp3^lo T_regP, and mature T_reg from Foxp3^eGFP (n = 9) (c) and Foxp3^eGFPxLta^−/− (n = 8) (d) mice. Graphs show the percentage of glucose dependence, mitochondrial dependence, glycolytic capacity as well as fatty acid and amino acid (FAO and AAO) capacity of each cell subset. Data are pooled from three independent experiments. e The table summarizes the phenotype of semi-mature (SM), mature 1 (M1) and mature 2 (M2) CD4⁺ SP thymocytes. f, g SCENITH metabolic profiling depending on maturational state of CD25⁺ T_regP, Foxp3^lo T_regP, and mature T_reg from Foxp3^eGFP (n = 9) and Foxp3^eGFPxLta^−/− (n = 6) mice. Data are pooled from three independent experiments. Error bars show mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 using one-way ANOVA for (a), (c) and (f), unpaired two-tailed Student’s t test for (b) and (d), unpaired two-tailed Mann–Whitney U test for (g). Source data are provided as a Source Data File.