Fig. 4: SARS-CoV-2 N protein represses miRNA biogenesis.
From: SARS-CoV-2 N protein-induced Dicer, XPO5, SRSF3, and hnRNPA3 downregulation causes pneumonia
See also Supplementary Fig. 5. a Heatmap of miRNA expression in A549-Ctrl and A549-N cells based on small RNA sequencing. b, c Quantification of five miRNAs in A549-Ctrl or A549-N cells (b) or A549-hACE2 cells infected with SARS-CoV-2 or mock (c). d Ratio of cytosolic levels of different RNAs to their nuclear levels in A549-Ctrl or A549-N cells. e Ratio of cytosolic levels of different RNAs to their nuclear levels in A549-hACE2 cells infected with SARS-CoV-2 or mock. f Ratio of cytosolic pre-miRNA levels to nuclear pre-miRNA levels in A549-Ctrl or A549-N cells transfected with a plasmid expressing XPO5 or control plasmid. g Ratio of cytosolic pre-miRNA levels to nuclear pre-miRNA levels in A549-hACE2 cells transfected with a plasmid expressing XPO5 or control plasmid and infected with SARS-CoV-2 or mock. h Ratio of mature miRNA levels to pre-miRNA levels in A549-Ctrl and A549-N cells. i Ratio of mature miRNA levels to pre-miRNA levels in A549-hACE2 cells infected with SARS-CoV-2 or mock. j Ratio of mature miRNA levels to pre-miRNA levels in A549-Ctrl and A549-N cells transfected with a plasmid expressing Dicer or a control plasmid. k Ratio of mature miRNA levels to pre-miRNA levels in A549-hACE2 cells transfected with a plasmid expressing Dicer or a control plasmid and infected with SARS-CoV-2 or mock. l miRNA levels in A549-Ctrl or A549-N cells co-transfected with XPO5-expressing and Dicer-expressing plasmids. m miRNA levels in A549-hACE2 cells transfected with XPO5-expressing and Dicer-expressing plasmids or control plasmid and infected with SARS-CoV-2 or mock. Data in (b–m) are expressed as mean ± SD of three biological replicates. **p < 0.01; *p < 0.05; ns not significant (p > 0.05; two-tailed unpaired Student’s t-test). Ctrl control plasmid, N N protein, siNC negative control siRNA. Source data and exact p values are provided in the Source Data file.
