Fig. 8: Age-related downregulation of Dicer, XPO5, SRSF3, and hnRNPA3 is associated with the severity of N protein-induced pneumonia.

See also Supplementary Fig. 8. a Expression levels of Dicer, Xpo5, Srsf3, and Hnrnpa3 in the lung tissues of 3-, 6-, 12-, and 24-month-old mice. Data were downloaded from the Gene Expression Omnibus (GEO) database (GSE209891) and are expressed as mean ± SD of three mice per group (two-tailed unpaired Student’s t-test). b, c mRNA (b) and protein (c, n = 5 mice per group) levels of Dicer, XPO5, SRSF3, and hnRNPA3 in the lung tissues of 8-week-old and 18-month-old mice. d, e Mice (8-week-old and 18-month-old) were intranasally instilled with control or N protein-expressing plasmid and subjected to immunoblotting with the indicated antibodies (d) and HE staining to assess lung injury (e). f, g Eight-week-old mice were intranasally instilled with N protein-expressing plasmid or co-instilled with N protein-expressing plasmid and shRNA-expressing plasmids, and their lung tissues were subjected to immunoblotting with the indicated antibodies (f) and HE staining to assess lung injury (g). The numbers below the blots (c, d, f) indicate the relative densitometric quantification of the bands normalized to corresponding GAPDH bands. Representative immunoblots of lung tissues from three out of five mice per group are shown (d, f). Data in (b, e, g) are expressed as mean ± SD of five mice. **p < 0.01; *p < 0.05; ns not significant (p > 0.05; two-tailed unpaired Student’s t-test). Ctrl control plasmid, N N protein, shNC negative control shRNA, sh-all mice instilled with shDicer, shXPO5, shSRSF3, and shhnRNPA3 together. Source data and exact p values are provided in the Source Data file.