Fig. 1: Aging induces hyper-metabolic B cells.
From: Immunometabolic effects of lactate on humoral immunity in healthy individuals of different ages
B cells, isolated from the peripheral blood of young and elderly individuals using magnetic beads, were left unstimulated. B cells were seeded into the wells of an extracellular flux analyzer at the concentration of 2 × 105/well in triplicate and run in a mitostress test. a OCR results representative of 5 independent experiments. b Maximal OCR. c Spare respiratory capacity (calculated subtracting basal OCR from maximal OCR values). d ECAR results representative of five independent experiments. e Maximal ECAR. f OCR:ECAR at maximal respiration. Resuls show mean ± Standard Error (SE) (with the exeption of the experiments shown in a and d. Source data are provided as a Source Data file. Mean comparisons between groups were performed by unpaired Student’s t test (two-tailed). *p < 0.05, **p < 0.01, ****p < 0.0001.
