Fig. 5: Pulmonary Wnt signaling activates CCL1 expression to attract γδ T cells after sepsis.

a Heatmap showing the chemokine expression signature in sham-operated and CLP mice (n = 4). b The protein levels of CCL1 in the murine lung after combined prophylactic and therapeutic administration of S-KT or 0.9% saline (n = 6). c Transwell assay was used to assess the ability of CCL1 to chemotaxis sorted-γδ T cells from spleen mononuclear cells (n = 6). d CCR8 expression by intestinal γδ T cells in sham-operated and CLP mice was measured by FCM analysis (n = 6 for sham, 7 for CLP). Sorted-γδ T cells from spleen mononuclear cells in sham-operated or CLP mice were co-cultured with AMs. Expression of e IL-17A and f IFN-γ by γδ T cells was measured by FCM analysis (n = 3). g Heatmap of the top 29 up-regulated DEGs in the lung between the sham-operated mice and CLP mice (n = 3). h The protein levels of the components of the Wnt signaling pathway (Wnt5a, FZD5, β-catenin, and lymphoid enhancer-binding factor 1 (Lef1)) in murine lungs after combined prophylactic and therapeutic administration of S-KT or 0.9% saline (n = 6). i Average plots and heat maps showing the signals at the TSS in the ChIP-seq data sets. j Pie charts showing the distribution of all Lef1 peak locations. k Distal intergenic region-specific binding peaks between Lef1 and CCL1 gene. Data are shown as mean ± SD. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001. ns not significant. P values were calculated using one-way ANOVA followed by Tukey’s multiple-comparison test (b, h), and two-sided Student’s unpaired t-test (c–f). Source data and exact P values are provided as a Source Data file.