Fig. 2: Regulation of distal germ cell behavior.
From: A nucleic acid binding protein map of germline regulation in Caenorhabditis elegans
a Quantification of nuclei number in the PZ of 3xflag::sygl-1; sun-1p::rde-1; rde-1(mkc36) one-day adults. n = 24. Green dashed line = average of the control. P values assessed by two-tailed multiple unpaired t test with no correction. b Quantification of nuclei number of one-day adult germline PZ of sun-1p::rde-1; rde-1(mkc36) treated with control RNAi and glp-1(ar202); sun-1p::rde-1; rde-1(mkc36) treated with control or experimental RNAi. n = 31, 31, 31, 31, 29, 32, 37, 32, 33, 30, 34, 31 and 31. P values assessed by one-way analysis of variance (ANOVA) with no correction for multiple comparisons. c Quantification of nuclei number of one-day adult germline PZ of sun-1p::rde-1; rde-1(mkc36) treated with control RNAi and glp-1(e2141); sun-1p::rde-1; rde-1(mkc36) treated with control or experimental RNAi. n = 30. P values assessed by one-way analysis of variance (ANOVA) with no correction for multiple comparisons. d, e Quantification of SYGL-1+ nuclei number (d) and confocal micrographs of germline PZ (e) of 3xflag::sygl-1; sun-1p::rde-1; rde-1(mkc36) one-day adults. n = 24. Green dashed line = average of the control (d); yellow dashed line = PZ/TZ boundary (e–left) and white line = SYGL-1 boundary (e–right). P values assessed by two-tailed multiple unpaired t test with no correction. f Proliferation rates of the C. elegans germ line after GNBP RNAi. RNAi was performed in triplicate from the L1 stage for 66 hrs before commencing EdU labeling, and germ lines were collected and imaged after 4 and 10 hrs. The 3xflag::sygl-1; sun-1p::rde-1; rde-1(mkc36) strain was used in this experiment. Data for each replicate shown; n = 63, 61, 63, 61, 62, 61, 58, 61, 63, 62, and 61 germ lines for each condition. P values assessed by two-tailed multiple unpaired t test with no correction. g Confocal micrographs showing DAPI and EdU+ nuclei after 4 and 10 hrs of EdU labeling. All RNAi was performed from the L1 stage. All Data was generated from three independent experiments, and results were normalized to respective controls in a–d. Error bars indicate SEM. Scale bars = 20 μm. Source data are provided as a Source Data file.
