Fig. 3: Regulation of meiotic germ cell behavior.
From: A nucleic acid binding protein map of germline regulation in Caenorhabditis elegans
a Quantification of multinucleated germ cells of pie-1p::mCherry::his-58; pie-1p::GFP::PH(PLC1δ1); sun-1p::rde-1; rde-1(mkc36) two-day adults following RNAi from the L1 stage. n = 30, 30, 31, 30, 31, 33, 30, 30, 30, 30 and 30. P values assessed by one-way ANOVA with no correction for multiple comparison. b Fluorescence micrographs of wild-type or ced-3(rp190) two-day adult germ lines in the pie-1p::mCherry::his-58; pie-1p::GFP::PH(PLC1δ1); sun-1p::rde-1; rde-1(mkc36) strain. Control RNAi and RNAi of dmd-7 and baz-2 were applied from the L1 stage in wild-type animals. Dash lines = border between the TZ (above) and pachytene region (below) of the germ line. Asterisks = multinucleated cells; triangles = apoptotic cells. c, d Quantification of apoptotic germ cells (c) and multinucleated germ cells (d) in two-day adults of pie-1p::mCherry::his-58; pie-1p::GFP::PH(PLC1δ1); sun-1p::rde-1; rde-1(mkc36) strain (wild-type and ced-3(rp190) animals). n = 30 (c), 30 and 33 (d). P values assessed by two-tailed unpaired t test. e Quantification of multinucleated germ cells in ced-3(rp190), pie-1p::mCherry::his-58; pie-1p::GFP::PH(PLC1δ1); sun-1p::rde-1; rde-1(mkc36) two-day adults following RNAi from the L1 stage. n = 30. P values assessed by one-way ANOVA with no correction for multiple comparison. f, g Confocal micrographs of DAPI (white) and phalloidin (pink) staining (f) and quantification of germline folding events in the pachytene region (g) of sun-1p::rde-1; rde-1(mkc36) one-day adults. Images for control RNAi (L4440) and lin-26 RNAi are shown in f (note—only one plane is shown). Yellow dashed line = border between the TZ (right) and pachytene region (left) of the germ line. n = 36, 29, 31, 24, 29, 32, 29, 24, 23, 28 and 26. P values assessed by one-way ANOVA with no correction for multiple comparison. All RNAi was performed from the L1 stage. Data was generated from three (a, c–e) or four independent experiments (g). Error bars indicate SEM. Scale bars = 20 μm. Source data are provided as a Source Data file.
