Fig. 2: The regulatory network of hypermucoviscosity includes many sRNAs. | Nature Communications

Fig. 2: The regulatory network of hypermucoviscosity includes many sRNAs.

From: RNA interactome of hypervirulent Klebsiella pneumoniae reveals a small RNA inhibitor of capsular mucoviscosity and virulence

Fig. 2

The sRNA-mRNA interaction network involving genes in the capsule biosynthesis cluster (a) and known hypermucoviscosity-regulating genes outside the capsule cluster (b). See also Supplementary Fig. 7b and Supplementary Data 6. c The impact of sRNA overexpression on hypermucoviscosity. Strains containing sRNA expression plasmids were grown overnight in LB containing 1 mM IPTG. Cultures were centrifuged at 1000 g for 5 min, and OD600 of supernatant was measured and normalized to the OD600 of the culture before centrifugation. Relative HMV values are shown relative to the basal HMV level of WT containing a pJV300 control vector. N = 3 biological replicates, bars indicate mean ± SD. *, p < 0.05; **, p < 0.01; two-tailed Student’s t test. d The ArcZ sRNA reduces HMV in different model and clinical strains. Shown in bold and underlined are two clinical CR-HvKP isolates RJ9299 and 1219 (Supplementary Data 7). N = 3 biological replicates, bars indicate mean ± SD. *, p < 0.05; **, p < 0.01; two-tailed Student’s t test.

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