Fig. 4: ArcZ represses mlaA and fbp via direct basepairing. | Nature Communications

Fig. 4: ArcZ represses mlaA and fbp via direct basepairing.

From: RNA interactome of hypervirulent Klebsiella pneumoniae reveals a small RNA inhibitor of capsular mucoviscosity and virulence

Fig. 4

Browser images showing the stacked chimeric ArcZ reads mapped in the mlaA (a) or fbp (b) loci. The y-axis shows the normalized reads (CPM: counts per million); S-chimera track: Fragments of mlaA or fbp mRNAs detected in ArcZ S-chimeras in Hfq libraries. Predicted base pairing for ArcZ with mlaA (c) and fbp (d). Shine-Dalgarno sequences are shown in red. Numbers indicate the position relative to the start codon of mRNA and numbers below indicate the position in ArcZ. The introduced mutations are indicated. Compensatory basepair exchange analysis to confirm direct interactions with mlaA (e) and fbp (f). Fluorescence levels were analyzed for strains expressing MlaA-GFP and Fbp-GFP reporters. N = 3 biological replicates, bars indicate mean ± SD.

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