Fig. 1: Expected gene drive haplotypes among offspring from an F1 gene drive/wild type strain hybrid, according to the mechanism of biased inheritance.
A Single nucleotide polymorphisms (SNPs) around the gene drive site between the F1 parent chromosomes allow identification of the parent chromosome inherited by each offspring, as well as the presence and amount of resection produced during gene drive homing via SNP presence or absence. Regions between present and absent SNPs are labelled ambiguous as there is no way to determine the origin of these sequences, or where conversion tracts produced during homing end within these sequences. An absence of any offspring containing the gene drive element on the alternate background indicates inheritance is biased by a meiotic drive mechanism, whereby the alternate chromosome is cut and destroyed. B Crosses of F1 hybrids used to produce offspring pools: Pools A-F were collected from zpg−7280/N’Gousso hybrids, with the female parent of each pool also collected. Pools G-L were collected from individual WT females mated en masse to vas2−5958/N’Gousso hybrid males (since females of this line are sterile); therefore, samples of the gene drive parent could not be taken. Figure 1 was created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en).
