Table 1 Mosquito strains used for crosses

From: Homing gene drives can transfer rapidly between Anopheles gambiae strains with minimal carryover of flanking sequences

Wild type strains

Strain

Species

Place and date of collection

Refs.

G3

An. gambiae/An. coluzzii hybrid

The Gambia, 1975

11

N’Gousso

An. coluzzii

Yaoundé, Cameroon, 2002

34

Gene drive lines

Line

Locus/target

Strain

Cassette

Refs.

vas2-5958

AGAP005958 (yellow-g)

G3

vas2-Cas9, U6-gRNA, 3xP3-RFP

11

zpg−7280

AGAP007280 (nudel)

G3

zpg-Cas9, U6-gRNA, 3xP3-RFP

21

  1. All gene drive lines were created by and obtained from Hammond et al. (2016 and 2021); the G3 strain was obtained from Imperial College London, and the N’Gousso strain obtained from Liverpool School of Tropical Medicine.
Back to article page