Fig. 1: Every organ has a sexual identity.

a Diagrams displaying the genomic loci of tra, fru and dsx genes. A blue and red triangle indicates the male- and female-specific stop coding in the tra locus. b Expression pattern of dsx-Gal4 and fru-Gal4 (P1 promoter) reporters in female tissues at the third larval stage (DNA labelled with DAPI, blue; the membrane-bound form of GFP, green). c Expression pattern of tra promoter-Gal4 reporter in male and female tissues at the third larval stage (DNA labelled with DAPI, blue; nucleus-targeted GFP, green). d Expression pattern of traF-Gal4 reporter in male and female tissues at the third larval stage (DNA labelled with DAPI, blue; nucleus-targeted GFP, green). e Expression pattern of traF-Gal4 reporter, combined with the dsx-Gal80 and fru-Gal80 (P1 promoter) repressors, in female tissues at the third larval stage (DNA labelled with DAPI, blue; the nuclear form of GFP, green). The visualised cell populations and specific organs are green in the right and top diagrams. Diagrams of the new alleles generated are presented at the top of each panel. Immunohistochemical analyses were repeated at least three independent times.