Fig. 5: Cellular sex requires the splicing activity of TraF and its cofactor Tra2.

a Sex transformations of abdominal pigmentation and genitalia induced by tra knock-out and RS domain mutation. b–d Adult wing size (b), adult weight (c), and female fertility (d) quantifications following RS domain mutation. e Expression pattern of tra2-Gal4 reporter in the female tissues at the third larval stage (DNA labelled with DAPI, blue; the nuclear form of GFP, green). f Diagrams representing the specific cell population with tra2 knock-out, the alleles generated, and the genotype used. g–i Adult wing size (g), adult weight (h), and female fertility (i) quantifications following tra2 knock-out specifically in dsx- and fru-negative cells. Data was combined from at least three independent experiments. n = wing number measured per genotype in (b) and (g), number of replicates (each repeat containing five flies) in (c) and (h) and number of flies in (d) and (i). Asterisks highlighting significant comparisons across sexes are displayed in grey boxes at the bottom of graphs; those highlighting significant comparisons within female datasets are displayed in red. For all panels, p-values from one-sided Mann-Whitney-Wilcoxon tests are ***p < 0.0001, (ns) p > 0.99.