Fig. 6: Downregulation of PGK1 crotonylation promotes cell proliferation and tumorigenesis.

a Reconstituted expression of the rPGK1 WT or 3KR proteins was performed in endogenous PGK1-deleted MDA-MB231 cells. The expression of PGK1 was detected by immunoblotting. b, c The colony formation of MDA-MB231 cells with or without PGK1 deletion and with or without reconstituted expression of rPGK1 WT or 3KR. The images (b) and counted colony numbers (c) of indicated groups were shown. d Cell proliferation of MDA-MB231 cells with or without PGK1 deletion and with or without reconstituted expression of rPGK1 WT or 3KR. e, f Cell invasion ability of MDA-MB231 cells with or without PGK1 deletion and with or without reconstituted expression of rPGK1 WT or 3KR. The images (e) and counted invasive cell numbers (f) of indicated groups were shown. g, h Cell migration of MDA-MB231 cells with or without PGK1 deletion and with or without reconstituted expression of rPGK1 WT or 3KR. The images (g) and relative migration distance(h), were shown. i, j MDA-MB231 cells with or without PGK1 deletion and with or without reconstituted expression of rPGK1 WT or 3KR were subcutaneously injected into nude mice. Tumor volume (i) and tumor formation (j) were measured. k PDHK1 and PGK1 expression in MDA-MB231/rPGK1 WT cells, MDA-MB231/rPGK1 3KR cells with or without PDHK1 depletion. l Cell proliferation of MDA-MB231/rPGK1 WT cells, MDA-MB231/rPGK1 3KR cells with or without PDHK1 depletion. m–o MDA-MB231/rPGK1 WT cells, MDA-MB231/rPGK1 3KR cells with or without PDHK1 depletion were subcutaneously injected into nude mice. Tumor formation (m), tumor volume (n), and tumor weight (o) were measured. In c, d, f, h, i, l, n, o, data are shown as the mean ± SD, n = 3 (c, d, f, h, l) or 6 (i, n, o) biologically independent samples. P values were obtained using unpaired two-tailed Student’s t test (c, f, h, i, n, o) or two-way ANOVA with Sidak test (d, l). Data were verified in at least two (i, j, m–o) or three (a–h, k, l) independent experiments. Source data are provided as a Source Data file.