Fig. 7: Low K131 crotonylation of PGK1 indicates a poor prognosis in breast cancer patients.

a–g IHC staining (a) and the quantitative results (b–g) of PGK1 K131cr, PGK1, ECHS1, p-PDHK1, t-PDHK1 and HIF-1α expression in the normal breast tissues from wild-type (FVB) mice and tumor tissues from the MMTV-PyMT mice with early (week 7) or late (week 18) stages of spontaneous breast cancer. Data are shown as the mean ± SD, n = 6 biologically independent samples (unpaired two-tailed Student’s t test). h Expression of PGK1 K131cr, PGK1 and ECHS1 were detected by immunoblotting in samples from the adjacent normal breast tissues (N) and breast tumor tissues (T) using the indicated antibodies. i, j IHC staining (i) and the quantitative results ( j) of PGK1 K131cr expression in samples from adjacent normal breast tissues and breast tumor tissues. Data are shown as the mean ± SD, n (N) = 6 and n (T) = 15 biologically independent samples (unpaired two-tailed Student’s t test). k Kaplan–Meier survival curve showing the overall survival of breast cancer patients according to the levels of PGK1 K131cr expression detected by IHC staining. n (high, 3–4 staining degree) = 70, n (low, 0–2 staining degree) = 59 (log-rank). l PGK1 K131cr expression in breast tumor tissues from patients at different grades (left) or from patients with different lymph node-positive rates (right). m IHC staining of ECHS1 in samples from adjacent normal breast and breast tumor tissues. n Kaplan–Meier survival curve depicting the overall survival of breast cancer patients based on ECHS1 expression in the TCGA cohort. n (high) = 749, n (low) = 287 (log-rank). Source data are provided as a Source Data file.