Fig. 1: Genome-scale CRISPR/Cas9 screen identifies IR resistance genes. | Nature Communications

Fig. 1: Genome-scale CRISPR/Cas9 screen identifies IR resistance genes.

From: PD-L1 deglycosylation promotes its nuclear translocation and accelerates DNA double-strand-break repair in cancer

Fig. 1

a Schematic illustration of the CRISPR/Cas9 loss-of-function screen in human lung cancer H460 cells. b Volcano plot showing both negatively (left) and positively (right) selected genes at day 7 after radiation treatment (4 Gy). CD274 gene that encodes PD-L1 is highlighted in green. c Negative selection of CRISPR screen data ranked based on RRE method. d Differentially selected sgRNAs from (b) were subject to a functional enrichment analysis using over-representation test (ORT) as implemented in MAGeCKFlute.

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