Fig. 3: Radiation induces PD-L1 nuclear localization through deglycosylation. | Nature Communications

Fig. 3: Radiation induces PD-L1 nuclear localization through deglycosylation.

From: PD-L1 deglycosylation promotes its nuclear translocation and accelerates DNA double-strand-break repair in cancer

Fig. 3

a H460 cells expressing GFP-tagged PD-L1 were treated with 4 Gy of IR at 2 Gy/min dose rate, followed by time-lapse imaging of GFP-PD-L1 in living cells. The pictures were captured every 5 min. b H460 cells were treated with 4 Gy of IR at 2 Gy/min dose rate, followed by isolation of cytoplasmic and nuclear fractions. PD-L1 was analyzed by western blot. α-Tubulin or PCNA was used as cytoplasmic or nuclear marker, respectively. **PD-L1: glycosylated form, *PD-L1: nonglycosylated form. c H460 cells expressing Flag-tagged PD-L1 were treated with glycosylation inhibitor tunicamycin (5 µg/ml) for various times, followed by immunofluorescence staining with anti-Flag antibody. d Schematic representation of the N → Q mutation at single- or multi-glycosylation site(s) in PD-L1. e, f Flag-tagged WT and various PD-L1 mutants were transfected into H460 cells, followed by western blot or immunofluorescence staining using anti-Flag antibody. **PD-L1: glycosylated form, *PD-L1: nonglycosylated form. Source data are provided as a Source Data file.

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