Fig. 4: CMTM6 directly interacts with and transports PD-L1 into the nucleus. | Nature Communications

Fig. 4: CMTM6 directly interacts with and transports PD-L1 into the nucleus.

From: PD-L1 deglycosylation promotes its nuclear translocation and accelerates DNA double-strand-break repair in cancer

Fig. 4

a PD-L1/CMTM6 interactions were analyzed by PLA at various times following IR exposure in H460 cells. PLA signals were detected by fluorescence microscopy as discrete spots. IgG, single PD-L1 antibody alone or single CMTM6 antibody alone was used as negative control. After PLA, immunofluorescence staining using α-tubulin antibody was performed as phase contrast imaging. Error bars represent ± s.d., n = 10 per group. ****P < 0.0001, by two-tailed t test. b PD-L1/CMTM6 interactions were analyzed by co-IP using PD-L1 antibody following IR exposure in H460 cells. **PD-L1: glycosylated form, *PD-L1: nonglycosylated form. c Ctrl siRNA or CMTM6 siRNA was transfected into H460 cells, followed by western blot using CMTM6 antibody. d H460 cells expressing Ctrl siRNA or CMTM6 siRNA were treated with IR (4 Gy), followed by immunofluorescence staining with PD-L1 antibody. Source data are provided as a Source Data file.

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