Fig. 2: Structural basis for the DIM2-HP1 interaction.
From: Multi-layered heterochromatin interaction as a switch for DIM2-mediated DNA methylation
a, b Close-up view of the interaction between DIM2 and HP1 homodimer, with the two HP1 CSD subunits shown in surface view (HP1-I: hot pink, HP1-II: magenta) and DIM2 shown in ribbon representation. c Close-up view of the interaction between HP1 and DIM2 at interface I. d Close-up view of the interaction between HP1 and DIM2 at interface II. e Close-up view of key HP1-contacting residues of DIM2, with HP1 shown in electrostatic surface representation. f Size-exclusion chromatography analysis of DIM2, WT or mutant, mixed with HP1. g In vitro DNA methylation assay of DIM2, WT or mutant, on a (CTA)12/(TAG)12 DNA duplex, in the presence of HP1 and histone H31-24K9me3 peptide. LLR, DIM2 L134A/L139A/R1104A triple mutation. Data are mean ± s.d. (n = 3 biological replicates). Statistical analysis used two-tailed Student’s t test. ***p = 0.0003; ****p < 0.0001. Source data are provided as a Source Data file.
