Fig. 5: Structural basis of the DIM2-DNA interaction.
From: Multi-layered heterochromatin interaction as a switch for DIM2-mediated DNA methylation
a The overall view of intermolecular interactions between DIM2 (cyan) and DNA (purple), with individual DNA-binding regions of DIM2 delimited by residue numbers. Close-up view of the DNA interactions by the catalytic loop (b), bridging loop (c), RFTS (d), and TRD (e). The hydrogen bonds are depicted as red dashed lines. f In vitro DNA methylation assay of DIM2, WT or DNA binding-associated mutant, on a (CTA)12/(TAG)12 DNA duplex. The corresponding regions of DIM2 are indicated on top. Data are mean ± s.d. (n = 3 biological replicates). Statistical analysis used two-tailed Student’s t test. ****p < 0.0001. Source data are provided as a Source Data file.
