Fig. 6: Structural basis for H3K9me3-mediated DIM2 activation.
From: Multi-layered heterochromatin interaction as a switch for DIM2-mediated DNA methylation
a Close-up view of the interaction of the H31-24K9me3 peptide with the DIM2 RFTS domain and DNA. The hydrogen bonds are depicted as dashed lines. b Close-up view of the interaction between the H31-24K9me3 peptide and the DIM2 BAH1 domain. c ITC binding curves for DIM2, WT or mutant, with the H31-24K9me3 peptide. The average and error estimates of the dissociation constants (Kd) were derived from two independent measurements. NDB no detectable binding. d In vitro DNA methylation assay of DIM2, WT or H31-24K9me3 binding-associated mutant, on a (CTA)12/(TAG)12 DNA duplex. Data are mean ± s.d. (n = 3 biological replicates). Statistical analysis used two-tailed Student’s t test. ***p = 0.0003; ****p < 0.0001. Source data are provided as a Source Data file.
