Fig. 1: Overview of single-cell long-read targeted sequencing.
From: Single-cell long-read targeted sequencing reveals transcriptional variation in ovarian cancer
Ovarian cell lines or dissociated tumor cells are processed using droplet-based single-cell RNA-seq 3’-Gene expression assay to obtain cDNA. Targeted enrichment is performed followed by nanopore sequencing, cell barcode (CB) and unique molecular identifier (UMI) assignment, and read alignment. Downstream analysis enables the measurement of isoforms, SNVs, allelic expression, TCR sequences and gene fusions at single-cell resolution. Figure Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en).
