Fig. 2: Targeted long-read single-cell cDNA sequencing optimization and scTaILoR-seq performance.
From: Single-cell long-read targeted sequencing reveals transcriptional variation in ovarian cancer
a Schematic detailing library preparation methods (targeted, targeted+AM, and targeted+R2C2) tested for enrichment using long-read (LR) sequencing. b Complete reads (left), TSO-TSO artifacts (middle), and number of passed reads (QScore ≥7) (right) across library preparation methods. Targeted (n = 3 replicates), Targeted+AM (n = 2 replicates), Targeted+R2C2 (n = 2 replicates). Bars represent mean values across replicates. c Pseudobulk gene-level expression between short-read (SR) untargeted and scTaILoR-seq approach. CPM counts per million. d Metagene max-normalized coverage profiles for untargeted and targeted SR and LR sequencing approaches. e Pseudobulk gene expression correlation for scTaILoR-seq replicates. f Frequency distributions of read counts per gene for untargeted LR and scTaILoR-seq. g Number of genes and transcripts uniquely detected (single dot) or shared (‘joined’ dots) across untargeted LR sequencing and scTaILoR-seq. h Pseudobulk transcript-level expression between untargeted LR and scTaILoR-seq methods. i Number of observed on-target novel transcript models for untargeted LR sequencing and scTaILoR-seq. j Number of on-target fusions identified for untargeted LR sequencing and scTaILoR-seq. Source data are provided as a Source Data file.
