Fig. 5: Sequence, structural and kinase specificity context of phosphosites.

A Two-dimensional representation of all phosphosites and their 5-amino acid flanking sequences, excluding the central amino acid from the comparison. Each cluster has been manually selected to emphasize the densest regions. B Sequence logo plot for all clusters depicted in (A). C Distribution of confidence scores for all amino acids, specifically S/T/Y, and for phosphorylated S/T/Y detected across all tissues. Source Data are provided as a Source Data file. D Our mouse phosphoproteome data derived from nine tissues was applied to the kinome atlas search tool. E All phosphorylation sites detected in our study are plotted on the x-axis, sorted by the number of kinases that scored higher than 90 for a specific site. F Z-score transformed difference between abundances of shared phosphorylation sites in brain and liver tissue. Vertical dashed lines indicate thresholds for selection of phosphorylation sites that are used for kinase motif enrichment analysis. A chi-squared test was used to calculate p values after applying Haldane’s correction. G Based on the top sites per tissue, a motif enrichment analysis was performed and the resulting frequency of how often a kinase was predicted to act on a site was plotted on the x-axis, along with the p value on the y-axis. The scheme and types of analyses have been adapted from ref. ³⁸. See Supplementary Data 4 for analysis results.