Fig. 6: Mitochondrial phosphoproteomics reveals novel liver-specific phosphorylation site.

A Bar plot of all detected phosphorylation sites in our study stratified into categories that are directly derived from the PhosphoSitePlus database (downloaded August 22, 2023). B Mouse MitoCarta 3.0⁸² proteins with at least one detected phosphorylation site in our data versus the remainder. C Bar plot indicating the number of mitochondrial phosphorylation sites that occur in a specific number of tissues. D Intersections of phosphorylation sites on mitochondrial proteins per tissue subsets. Intersection sizes of 12 or more are shown. Sub-mitochondrial localization is derived from MitoCarta 3.0. E Dot plot displaying all proteins that harbor phosphorylation sites unique to liver tissue. The highest ranked protein harbors the most phosphorylation sites as indicated on the x-axis. Novel sites are according to the PhosphoSitePlus database. F Schematic representation of the CPS1 peptide chain with all detected phosphorylation sites indicated as novel or previously identified. G Sequence alignment of CPS1 orthologues using Clustal Omega⁸¹. Patient variant residue and phosphorylation site residue of interest are in gray. H Structural modeling based on structures (PDB: 5DOT (Apo), 5DOU (NAG)) from RCSB PDB⁶⁶. Source Data are provided as a Source Data file.