Fig. 3: The phylogenetic analysis and functional validations of Apiaceae C2’Hs.
From: The gradual establishment of complex coumarin biosynthetic pathway in Apiaceae
a The phylogeny of 2-OGD gene family from 154 plant genomes and 17 transcriptomes, see Supplementary Fig. 29 for the detailed phylogeny. GenBank accession numbers: PsC2’H, APP94171.1; PpC2’H, ASR80916.1; AtS8H, NP_187896.1 (AT3G12900.1); GmF6’H, AHY03267.1; AtF6’H1, NP_187970.1 (AT3G13610.1); AtF6’H2, NP_175925.1 (AT1G55290.1) b The extracted phylogeny of Apiaceae F6’H and C2’H. The green dot indicates the origin of C2’H function. The red and purple dots show that the corresponding gene has C2’H and F6’H function, respectively. The orange pentacle represents functionally characterized genes published already. c The C2’H activity assays of selected Apiaceae 2-OGD genes. d The F6’H activity assays of selected Apiaceae 2-OGD genes. The LC maps of standards and their corresponding chemical structures are shown in the figure. The retention time of umbelliferone and scopoletin was 20.75 and 19.49 min, respectively. Source data are provided as a Source Data file.
