Fig. 4: The phylogenetic analysis and functional validations of Apiaceae PTs.
From: The gradual establishment of complex coumarin biosynthetic pathway in Apiaceae
a The phylogeny of PT gene family from 154 plant genomes, see Supplementary Fig. 25 for the detailed phylogeny. b The extracted phylogeny of Apiaceae PTs. c Enzymatic activity assays of C-PTs by HPLC. d Enzymatic activity assays of O-PTs by HPLC. e Six significant discrepant amino acid sites between the U6- and U8PTs in Apiaceae. f Enzymatic activity assays of PpPT1 and PpPT2 with site-specific mutations by HPLC. DMAPP, dimethylallyl pyrophosphate; DMS, demethylsuberosin. g 3-D protein structures of PpPT1 and PpPT2, and hypothesized process of umbelliferone prenylation catalyzed by U6PT and U8PT, respectively. Essential sites (Thr161/Ala161) for functional variations were displayed in colors. Source data are provided as a Source Data file.
