Fig. 3: Cooperative stabilization network rewiring in PCI complexes.

A, B The protein stability-based interaction map of the proteasome lid (A) and CSN (B) complexes. Louvain network modularity analysis of the data is shown on the right. Proteins with PCI or MPN domains or without these domains are denoted as circles, diamonds, or triangles, respectively. C, D Protein stability analysis of the subunit indicated on the plot upon supplying the subunit indicated at right. E A schematic to summarize the inter-subunit physical associations in the proteasome lid and CSN complexes identified by previous mass spectrometry analyses and co-immunoprecipitation experiments. F Cooperative stabilizing network topologies for the three PCI complexes. Paralogous subunits are represented in the same color and denoted according to the Latin alphabet indicated at left. Arrows denote directional cooperative stabilization interactions, with line thickness proportionally reflecting the intensity of protein stabilization. Source data are provided as a source data file.