Fig. 6: Divergence of protein stability-guided modularity in LSm- and Sm-type complexes.

A Louvain two-nearest-neighbor analysis of LSm- and Sm-type complexes based on data shown in Fig. 5B. Nodes of the same color denote paralogous proteins, and black arrows represent directional cooperative stabilization interactions. The thickness of the arrow lines proportionally reflects the degree of protein stabilization. Subunits forming the same network cluster are surrounded by a light gray shadow. B, C Comparison of paralogous interactions within LSm and Sm complexes in terms of cooperative stabilization. Protein stability analysis by GPS assay of proteins indicated on top with or without co-expressing the proteins indicated at right. D Comparison of protein stability among various LSm and Sm proteins upon their low-level expression (from a single copy of the GPS reporter). GFP was tagged at the C-termini of LSm/Sm proteins. E Correlation analysis between the stability of unassembled LSm/Sm proteins and their degrees of stabilization by corresponding partners.