Fig. 6: Rescue of hemorrhage in Esco2fl/fl;Prrx1-CreTg/0 limbs by pifithrin-α treatment. | Nature Communications

Fig. 6: Rescue of hemorrhage in Esco2fl/fl;Prrx1-CreTg/0 limbs by pifithrin-α treatment.

From: Limb reduction in an Esco2 cohesinopathy mouse model is mediated by p53-dependent apoptosis and vascular disruption

Fig. 6

a Bar chart of normalized scores for top 10 drug candidates identified by EMUDRA. Drugs are ranked by score with more negative values indicating stronger association. b Gross morphology of Esco2fl/fl;Prrx1-CreTg/0 and Esco2fl/fl embryonic limb buds at E12.5 treated with either PBS (top) or pifithrin-α (bottom). Scale bar: 1 mm. c TUNEL assay co-stained with PECAM1 of Esco2fl/fl (n = 3) and Esco2fl/fl;Prrx1-CreTg/0 (n = 3) limbs treated with PBS (left) or pifithrin-α (right) at E12.5 in the transverse plane. Scale bar: 100 µm. d Immunofluorescent staining of Esco2fl/fl (n = 3) and Esco2fl/fl;Prrx1-CreTg/0 (n = 3) limbs treated with PBS (left) or pifithrin-α (right) for SOX9 and PECAM1 at E12.5 in the transverse plane. Scale: 100 µm. e Box and whisker plot showing the corrected total cell fluorescence (CTCF) of TUNEL+ signal between Esco2fl/fl (n = 3) and Esco2fl/fl;Prrx1-CreTg/0 (n = 3) limbs treated with PBS or pifithrin-α. In each box plot, the center line indicates the median, the edges of the box represent the first and third quartiles, and the whiskers extend to span a 1.5 interquartile range from the edges. Asterisk indicates a significant difference using a one-tailed, Student’s t-test (p ≤ 0.05; p = 0.001 for PBS comparisons, p = 0.341 for pifithrin-α comparisons, and p = 0.031 for Esco2fl/fl;Prrx1-CreTg/0 comparisons between PBS and pifithrin-α groups). Source data are provided with this paper.

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