Fig. 2: Enhanced performance of the mt-IDH1dx assay in tumor tissue across the glioma subtypes.
From: Clinical utility of a blood based assay for the detection of IDH1.R132H-mutant gliomas
a Schematic comparing the modalities routinely applied in clinical settings for determination of IDH1.R132H status versus the one-step laboratory developed Droplet Digital PCR platform, referred to as mt-IDH1dx ddPCR assay, for rapid, tumor tissue diagnostics. b mt-IDH1dx assay performance evaluated in tumor tissue cohort (N = 48) in comparison to clinical pathology testing across different histological subtypes: (c) Astrocytoma, Grade 2, 3 (n = 25, biological replicates), (d) Astrocytoma, Grade 4 (n = 12, biological replicates), and (e) Oligodendroglioma, Grade 2, 3 (n = 11, biological replicates). f–h Astrocytomas, Grade 4 category includes n = 3 GBM tumor tissue samples that were determined IDH1 mutant by the ddPCR assay. f–h Scatter-dot plots demonstrating the overall distribution (mean ± SEM, derived from the average of three technical replicates per study patient) of ddPCR derived: (f) IDH1.R132H mutant copies, (g) IDH1 wild-type copies, and (h) IDH1.R132H MAF % across the three IDH1.R132H mutant glioma subtypes. f–h Brown-Forsythe and Welch ANOVA test with multiple comparisons was performed to evaluate the statistical significance of observed differences: (f) Astrocytoma, Grade 2–3 vs Grade 4 (−264.9, p value 0.9371), Astrocytoma, Grade 2–3, vs Oligodendroglioma, Grade 2–3 (−1229, p value 0.0435), Astrocytoma, Grade 4 vs Oligodendroglioma, Grade 2–3 (−963.8, p value 0.3766) (h) Astrocytoma, Grade 2–3 vs Grade 4 (6.794, p value 0.6707), Astrocytoma, Grade 2–3, vs Oligodendroglioma, Grade 2–3 (−17.26, p value 0.0059), Astrocytoma, Grade 4 vs Oligodendroglioma, Grade 2–3 (−24.05, p value 0.0073). i, j Kaplan–Meier curves were evaluated among patients with IDH1 mutant glioma by stratifying the patient population using different parameters: (i) glioma subtype, and (j) IDH1.R132H tumor tissue MAF %, cut-off (≤38%, >38%). P-value was calculated using Log-Rank test. Source Data are provided as a Source Data file. Figure 2/panel A, B Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en).
