Fig. 2: AP2 is present in the SAM as it increases in height and width during floral transition.

a Pattern of protein accumulation of AP2::AP2:VENUS at the SAM in ap2-12 plants under continuous LDs. Each SAM is shown in longitudinal section. The outline of each acquired meristem and its peripheral organs is indicated with a dotted white line. Scale bar = 50 μm. b–c Quantification of AP2:VENUS concentration of fluorescence intensity (total fluorescence divided by volume) at the shoot apex from the tip (b) to 50 μm deep or (c) 25 μm deep in the basal direction in the ap2-12 mutant background during continuous LDs. The horizontal bars represent the median value for each time point. Significant differences among time points within each genotype were determined via one-way ANOVA (two-sided), followed by Tukey post-hoc comparisons (p < 0.05). Data sets that share a common letter do not differ significantly. d Normalized measurements of height and width of the SAMs from LD-grown plants in this study (data from 6 independent experiments were pooled). The point of maximum height of each genotype in each experiment was selected. Measurements were normalized by the median of that measurement in Col-0 in each experiment. The horizontal bars represent the median value for each genotype. The outlines of the dots are colored according to the measurement. The dots are colored according to the identity of primordia that were formed at the SAM periphery. Significant differences for each measurement among genotypes were determined via one-way ANOVA (two-sided), followed by Tukey post-hoc comparisons (p < 0.05). Data sets that share a common letter do not differ significantly. See Supplementary Data 7 for precise sample size and p-values of Mann-Whitney-Wilcoxon and ANOVA test. Source Data are provided as a Source Data file.