Fig. 5: Phosphoacceptors foster polar interactions upon NCC activation by kinases. | Nature Communications

Fig. 5: Phosphoacceptors foster polar interactions upon NCC activation by kinases.

From: Structural bases for Na+-Cl− cotransporter inhibition by thiazide diuretic drugs and activation by kinases

Fig. 5

a Phosphorylation of Thr55, Thr60, and Ser73 strengthens NTD-CTD and NTD-TMD association. b Weakening phosphorylation-induced interactions abolishes or reduces NCC ion transport rates, measured as the slopes of fluorescence change in the first 20 s. Each circle represents one kinetic measurement of a single sample in a 96-well plate. Unpaired one-tailed Student’s t-tests are used for statistical analyzes (n = 6; data are presented as mean value ± SD). c pThr55, pThr60, and pSer73 are docked into corresponding densities. Source data are provided as a Source Data file.

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