Fig. 7: NCC assumes multiple dimeric forms. | Nature Communications

Fig. 7: NCC assumes multiple dimeric forms.

From: Structural bases for Na+-Cl− cotransporter inhibition by thiazide diuretic drugs and activation by kinases

Fig. 7

a Superimposition of one subunit of NKCC1/bumetanide and two individual subunits of NCC/indapamide highlights that the loop connecting TM12 and scissor helix can assume distinct conformations. b, c The TM12/scissor helix loop associates with C-tail and ICL1 (b) and weakening these interactions reduces NCC ion transport rate (c). In c Cl− influx rates are measured as the slopes of fluorescence change in the first 20 s. Each circle represents one kinetic measurement of a single sample in a 96-well plate. Unpaired one-tailed Student’s t-tests are used for statistical analyzes (n = 12; data are presented as mean value ± SD). d The TM12/scissor helix loop can disengage from ICL1 and assumes an alternative conformation stabilized by interactions with CTD. Source data are provided as a Source Data file.

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