Fig. 1: Characterization of CD4-induced anti-sera in an alpaca. | Nature Communications

Fig. 1: Characterization of CD4-induced anti-sera in an alpaca.

From: Highly potent and broadly neutralizing anti-CD4 trimeric nanobodies inhibit HIV-1 infection by inducing CD4 conformational alteration

Fig. 1

a Experimental schedule of immunization. b Detection of CD4 protein with His tag (CD4-His) or rabbit Fc tag (CD4-rFc) by SDS-PAGE. c Evaluation of anti-sera titer after the 3rd and 4th immunization in an alpaca receiving CD4-rFc protein. The y-axis represents the absorbance at 450 nm, and the x-axis shows the anti-sera dilution fold. Anti-sera binding with CD4-His are labeled on the graph. The terms “3rd anti-sera” (blue line) and “4th anti-sera”(red line) refer to the anti-sera collected one week after the 3rd and 4th immunizations, respectively. “Pre-immune” (black line) refer to the sera from alpaca before immunization, which was taken as negative control. # represents the antiserum titer. d Neutralizing curve of the 4th anti-sera against the panel of 13 HIV-1 pseudoviruses of various subtypes. Mulv served as the control virus. e Summary of 50% inhibitory dilution (ID50) and 90% inhibitory dilution (ID90) values, along with the information of pseudoviruses tested in the neutralization assay presented in panel d. Data for (c) and (d) are depicted as mean ± SEM. Source data are provided as a Source Data file.

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