Fig. 1: scRNA-Seq characterization of photoreceptor differentiation defects in gl mutants.
From: Synergistic activation by Glass and Pointed promotes neuronal identity in the Drosophila eye disc
a UMAP dimensional plot of scRNA-Seq data from wild-type and gl60j white prepupal eye discs harmonized using the Seurat integration method. The same colors are used for equivalent clusters in the two conditions. Clusters corresponding to photoreceptor types and cone cells are labeled. Other labeled clusters are Ppn preproneural, MF morphogenetic furrow, SMW second mitotic wave, Diff. PR differentiated photoreceptors. b Percentage of total cell counts in these integrated clusters in wild-type (red) and gl mutant (green) conditions. c, d Monocle3 trajectory inferences of the photoreceptor clusters using the integrated UMAP and raw counts for wild-type (c) and gl (d). Pseudotime is shown on a batlow scale, beginning with gray at the roots and ending with pink for the most differentiated cells. e, g, i, k, m, o, q, s Feature plots of gene expression levels in wild-type and gl mutant eye discs, with gray indicating no expression and pink the highest expression. f, h, j, l, n, p, r, t Violin plots of gene expression levels in the indicated selected cell clusters, with wild-type in red and gl in green. e, f sens is still expressed in R8 in gl mutants; g, h the late R8 marker CG42458 is not expressed; i, j rough is still expressed in R2, R5, R3, and R4; k, l CG7991 is lost from R1–R6; m, n blanks is lost from R3 and R4; o, p Bar-H1 is lost from R1 and R6; q, r CAP is lost from R7; s, t the late marker CG34377 is strongly reduced in all photoreceptors.
