Fig. 2: Gl and RasV12 synergistically induce neuronal markers in the wing disc.
From: Synergistic activation by Glass and Pointed promotes neuronal identity in the Drosophila eye disc
a–l Wing discs in which clones overexpressing the indicated proteins are marked with GFP (green), stained for the neuronal markers Elav, a nuclear protein (b, e, h, k, red in a, d, g, j) and Futsch, a cytoplasmic protein (c, f, i, l, blue in a, d, g, j). Anterior is to the left and dorsal is up. a–c Gl overexpression does not induce either protein. d–f RasV12 induces clone overgrowth but not the neuronal markers. g–i Overexpression of both Gl and RasV12 strongly induces Elav and Futsch. j–l Overexpression of Gl and RasV12 in pntΔ88 mutant clones induces much less Elav and Futsch expression than Gl and RasV12 in wild-type clones. All discs were imaged in parallel with the same laser settings. Scale bars, 50 μm. m, n Quantification of Elav (m) or Futsch (n) intensity in these genotypes, normalized to the background. Co-expression of Gl and RasV12 showed significantly greater Elav and Futsch levels than all other genotypes. Error bars indicate mean ± SD. m p(Gl + Ras, Gl) = 0.0002, p(Gl + Ras, Ras) = 0.0009, p(Gl+Ras, Gl + Ras pnt) = 0.0003. n p(Gl + Ras, Gl) = 0.0009, p(Gl + Ras, Ras) = 0.0018, p(Gl+Ras, Gl + Ras pnt) = 0.0021, two-tailed t-test with Welch’s correction. n = 7 discs (UAS-Gl; UAS-RasV12); n = 9 discs (UAS-Gl&RasV12; UAS-Gl&RasV12, pnt). Source data are provided as a Source Data file.
