Fig. 3: Synergistic activation of a neuronal program by Gl and RasV12.
From: Synergistic activation by Glass and Pointed promotes neuronal identity in the Drosophila eye disc
a Heat map showing the log2-transformed expression levels of the 265 genes that were significantly upregulated in wing discs with clones expressing UAS-Gl and UAS-RasV12 compared to UAS-Gl or UAS-RasV12 alone by differential gene expression analysis and were attenuated in pnt mutant clones. b Volcano plot showing expression levels of genes that were significantly different with Gl and RasV12 co-expression compared to Gl expression alone. Genes with |log2 fold change| > 1 are shown in red and <1 in blue. Selected neuronal genes are labeled. p values were computed with the Wald test in DESeq2. c–h Transcript levels (RPKM, libraries normalized to GFP level) of the synergistically activated neuronal genes Syt4 (c), futsch (d), lz (e), trp (f), scrt (g), and CG12605 (h). Error bars indicate mean ± SD. n = 3 biological replicates of the RNA-Seq experiment. i Panther-based GO term analysis of the 265 genes. Many GO terms are associated with neuronal functions. j Pie chart showing the proportion of the 265 genes that have neuronal functions documented in FlyBase. Source data are in Supplementary Data 3.
